Thesis (Ph.D.) - University of Warwick, 1992.
|Statement||by Tak-Wah DominicChan.|
Since its invention in the late s, matrix-assisted laser desorption/ionisation–mass spectrometry (MALDI-MS) has been applied to a large number of analytical problems such as the analysis of proteins, glycoconjugates, polynucleotides and synthetic polymers. This article explains briefly the principle of MALDI and the instrumentation by: Shelley N. Jackson, Amina S. Woods, in The Encyclopedia of Mass Spectrometry, 1 Introduction. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a modification of laser desorption/ ionization mass spectrometry (LDI-MS that allows for the analysis and detection of intact high molecular weight molecules. In LDI-MS, a pulsed laser, usually an . MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization." The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength nm) and converts it to heat energy. A small part of the matrix (down to nm from the top outer surface of the. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective diagnostic tool for the identification of organisms routinely found in the.
Robert Bradshaw and Simona Francese tell us about “Matrix–assisted laser desorption ionisation tandem mass spectrometry imaging of small molecules from latent fingermarks“. Especially when looking at small molecules in fingermarks, isobaric species can be a problem and this has the potential to affect the outcome of any court case if not handled . Laser desorption/ionization mass spectrometry for direct profiling and imaging of small molecules from raw biological materials Sangwon Cha Iowa State University Follow this and additional works at: Part of theAnalytical Chemistry Commons, and thePlant Biology Commons. Online shopping from a great selection at Books Store. Since then, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) evolved into a rapid and highly reliable analytical tool for the characterization of a diverse collection of microbes encountered in the clinical laboratory (19, 20). Currently, a variety of analysis methods and MS instruments are available Cited by:
In this respect, electrospray ionization mass spectrometry (ESI-MS) (Ekeberg et al., ) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) . Surface-enhanced laser desorption/ionization (SELDI) is a soft ionization method in mass spectrometry (MS) used for the analysis of protein is a variation of matrix-assisted laser desorption/ionization (MALDI). In MALDI, the sample is mixed with a matrix material and applied to a metal plate before irradiation by a laser, whereas in SELDI, proteins of interest in Analytes: Biomolecules. Vorm O, Roepstorff P. Peptide sequence information derived by partial acid hydrolysis and matrix-assisted laser desorption/ionization mass spectrometry. Biological mass spectrometry. ; – Beavis RC, Chait BT. Cinnamic acid derivatives as matrices for ultraviolet laser desorption mass spectrometry of by: Chemical analysis for the characterisation of micro-organisms is rapidly evolving, after the recent advent of new ionisation methods in mass spectrometry (MS): electrospray (ES) and matrix-assisted laser desorption/ionisation (MALDI). These methods allow quick characterisation of micro-organisms, either directly or after minimum sample by: